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human anti dsg2 (R&D Systems)

Name: human anti dsg2
Brand: R&D Systems
Rating: 93 (13 reviews)

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R&D Systems human anti dsg2

(A) Schematic of desmosome structure. (B-D) Representative STED images of desmosomes showing a characteristic ‘railroad track’ pattern in (B) MCF7 WT cells, (C) MCF7 K19-KO cells, and (D) MCF7 K19-GfP cells. The desmosomes were immunolabeled for DPC (green) and <t>Dsg2</t> (red). Scale bar: 500 nm. (E) The boxed area from the representative STED images of MCF7 WT cells shows a closer look at an individual DP railroad track with Dsg2 between the two parallel DP plaques. Scale bar: 200 nm. (F) Line-scan analysis of DPC and Dsg2 fluorescence intensity (indicated by the dashed line in E). (G) Quantification of DPC-DPC distance from WT and K19-KO cells showing that desmosomes in WT cells are wider than K19-KO cells. In all boxplots, the box represents the 25th and 75th percentiles with the median indicated and whiskers reach 1.5 times the interquartile range (IQR), defined as the difference between the 25th and 75th percentiles. Data points outside the whiskers are shown as outliers. Number of datapoints (n) = 630 (WT), 505 (K19-KO); Number of replicates (N) = 3. Mann-Whitney’s U test; ***, P<0.001. (H) Quantification of DPC-DPC distance in K19-KO, WT, and K19-GFP cells. Desmosomes in WT and K19-GFP cells are wider than K19-KO cells. n = 505 (K19-KO), 630 (WT), 716 (K19-GFP); N = 3. Kruskal-Wallis Test, followed by Dunn’s multiple comparison Test; ***, P<0.001.

Human Anti Dsg2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 13 article reviews

human anti dsg2 - by Bioz Stars, 2026-02

93/100 stars

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1) Product Images from "Desmoplakin is a desmosomal mechanosensor"

Article Title: Desmoplakin is a desmosomal mechanosensor

Journal: bioRxiv

doi: 10.1101/2024.11.19.624364

Figure Legend Snippet: (A) Schematic of desmosome structure. (B-D) Representative STED images of desmosomes showing a characteristic ‘railroad track’ pattern in (B) MCF7 WT cells, (C) MCF7 K19-KO cells, and (D) MCF7 K19-GfP cells. The desmosomes were immunolabeled for DPC (green) and Dsg2 (red). Scale bar: 500 nm. (E) The boxed area from the representative STED images of MCF7 WT cells shows a closer look at an individual DP railroad track with Dsg2 between the two parallel DP plaques. Scale bar: 200 nm. (F) Line-scan analysis of DPC and Dsg2 fluorescence intensity (indicated by the dashed line in E). (G) Quantification of DPC-DPC distance from WT and K19-KO cells showing that desmosomes in WT cells are wider than K19-KO cells. In all boxplots, the box represents the 25th and 75th percentiles with the median indicated and whiskers reach 1.5 times the interquartile range (IQR), defined as the difference between the 25th and 75th percentiles. Data points outside the whiskers are shown as outliers. Number of datapoints (n) = 630 (WT), 505 (K19-KO); Number of replicates (N) = 3. Mann-Whitney’s U test; ***, P<0.001. (H) Quantification of DPC-DPC distance in K19-KO, WT, and K19-GFP cells. Desmosomes in WT and K19-GFP cells are wider than K19-KO cells. n = 505 (K19-KO), 630 (WT), 716 (K19-GFP); N = 3. Kruskal-Wallis Test, followed by Dunn’s multiple comparison Test; ***, P<0.001.

Techniques Used: Immunolabeling, Fluorescence, Comparison

(A) Schematic of the desmosome under tension. The N-terminal of DP transitions from a closed conformation to an open conformation. (B) Quantification of desmosome half-unit widths (Dsg2-DPC distance) from WT and K19-KO cells. n = 1260 (WT), 1010 (K19-KO); N = 3. Mann-Whitney’s U test; ***, P<0.001. The distances between Dsg2 and DPC is significantly greater in the WT compared to the K19-KO cells. (C) Quantification of desmosome half-unit widths (Dsg2-DPN distance) from WT and K19-KO cells. n = 692 (WT), 826 (K19-KO); N = 3. Mann-Whitney’s U test; ns, P>0.05. The Dsg2-DPN distance in both cell lines are similar. (D) The mean values from the results in B and C are summarized in the bar chart to compare the DP length (DPN-DPC distance) between the WT and K19-KO cells. The DP length is 66 nm for the WT and 33 nm for the K19-KO, indicating that DP extends 33 nm in the WT cells. (E) Dispase assay after 24 h plating. The confluent cell sheets are treated with 4 mM EGTA for 1 h. The images show the intact cell sheets for WT, K19-KO, and K19-GFP rescued cells before stress and fragmented cell sheets after applying mechanical stress. (F) Quantification of the dispase assay from K19-KO, WT, and K19-GFP cells. n = 12 (KO), 12 (WT), 9 (K19-GFP); N = 3. Kruskal-Wallis Test, followed by Dunn’s multiple comparison Test; ***, P<0.001. The K19-KO cell sheets show a greater number of fragments compared to WT cell sheets, while K19-GFP cell sheets generate a similar number of fragments as WT. This indicates that elongation of DP strengthens intercellular adhesion in WT and K19-GFP cells.

Figure Legend Snippet: (A) Schematic of the desmosome under tension. The N-terminal of DP transitions from a closed conformation to an open conformation. (B) Quantification of desmosome half-unit widths (Dsg2-DPC distance) from WT and K19-KO cells. n = 1260 (WT), 1010 (K19-KO); N = 3. Mann-Whitney’s U test; ***, P<0.001. The distances between Dsg2 and DPC is significantly greater in the WT compared to the K19-KO cells. (C) Quantification of desmosome half-unit widths (Dsg2-DPN distance) from WT and K19-KO cells. n = 692 (WT), 826 (K19-KO); N = 3. Mann-Whitney’s U test; ns, P>0.05. The Dsg2-DPN distance in both cell lines are similar. (D) The mean values from the results in B and C are summarized in the bar chart to compare the DP length (DPN-DPC distance) between the WT and K19-KO cells. The DP length is 66 nm for the WT and 33 nm for the K19-KO, indicating that DP extends 33 nm in the WT cells. (E) Dispase assay after 24 h plating. The confluent cell sheets are treated with 4 mM EGTA for 1 h. The images show the intact cell sheets for WT, K19-KO, and K19-GFP rescued cells before stress and fragmented cell sheets after applying mechanical stress. (F) Quantification of the dispase assay from K19-KO, WT, and K19-GFP cells. n = 12 (KO), 12 (WT), 9 (K19-GFP); N = 3. Kruskal-Wallis Test, followed by Dunn’s multiple comparison Test; ***, P<0.001. The K19-KO cell sheets show a greater number of fragments compared to WT cell sheets, while K19-GFP cell sheets generate a similar number of fragments as WT. This indicates that elongation of DP strengthens intercellular adhesion in WT and K19-GFP cells.

Techniques Used: Comparison

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Journal: bioRxiv

Article Title: Desmoplakin is a desmosomal mechanosensor

doi: 10.1101/2024.11.19.624364

Figure Lengend Snippet: (A) Schematic of desmosome structure. (B-D) Representative STED images of desmosomes showing a characteristic ‘railroad track’ pattern in (B) MCF7 WT cells, (C) MCF7 K19-KO cells, and (D) MCF7 K19-GfP cells. The desmosomes were immunolabeled for DPC (green) and Dsg2 (red). Scale bar: 500 nm. (E) The boxed area from the representative STED images of MCF7 WT cells shows a closer look at an individual DP railroad track with Dsg2 between the two parallel DP plaques. Scale bar: 200 nm. (F) Line-scan analysis of DPC and Dsg2 fluorescence intensity (indicated by the dashed line in E). (G) Quantification of DPC-DPC distance from WT and K19-KO cells showing that desmosomes in WT cells are wider than K19-KO cells. In all boxplots, the box represents the 25th and 75th percentiles with the median indicated and whiskers reach 1.5 times the interquartile range (IQR), defined as the difference between the 25th and 75th percentiles. Data points outside the whiskers are shown as outliers. Number of datapoints (n) = 630 (WT), 505 (K19-KO); Number of replicates (N) = 3. Mann-Whitney’s U test; ***, P<0.001. (H) Quantification of DPC-DPC distance in K19-KO, WT, and K19-GFP cells. Desmosomes in WT and K19-GFP cells are wider than K19-KO cells. n = 505 (K19-KO), 630 (WT), 716 (K19-GFP); N = 3. Kruskal-Wallis Test, followed by Dunn’s multiple comparison Test; ***, P<0.001.

Article Snippet: The following primary antibodies were used: human anti-DSG2 (MAB947, R&D systems), rabbit anti-DPC antibody (A303-356A, Bethyl Lab), rabbit anti-DPN antibody (25318-1-AP, Proteintech), chicken anti-GFP (600-901-215, Rockland), mouse anti-K19 antibody (A53-B/A2) (Santa Cruz Biotechnology), control mouse IgG (sc-2025) (Santa Cruz Biotechnology), and mouse anti-actin antibody (66009-1-Ig, Proteintech).

Techniques: Immunolabeling, Fluorescence, Comparison

Journal: bioRxiv

Article Title: Desmoplakin is a desmosomal mechanosensor

doi: 10.1101/2024.11.19.624364

Figure Lengend Snippet: (A) Schematic of the desmosome under tension. The N-terminal of DP transitions from a closed conformation to an open conformation. (B) Quantification of desmosome half-unit widths (Dsg2-DPC distance) from WT and K19-KO cells. n = 1260 (WT), 1010 (K19-KO); N = 3. Mann-Whitney’s U test; ***, P<0.001. The distances between Dsg2 and DPC is significantly greater in the WT compared to the K19-KO cells. (C) Quantification of desmosome half-unit widths (Dsg2-DPN distance) from WT and K19-KO cells. n = 692 (WT), 826 (K19-KO); N = 3. Mann-Whitney’s U test; ns, P>0.05. The Dsg2-DPN distance in both cell lines are similar. (D) The mean values from the results in B and C are summarized in the bar chart to compare the DP length (DPN-DPC distance) between the WT and K19-KO cells. The DP length is 66 nm for the WT and 33 nm for the K19-KO, indicating that DP extends 33 nm in the WT cells. (E) Dispase assay after 24 h plating. The confluent cell sheets are treated with 4 mM EGTA for 1 h. The images show the intact cell sheets for WT, K19-KO, and K19-GFP rescued cells before stress and fragmented cell sheets after applying mechanical stress. (F) Quantification of the dispase assay from K19-KO, WT, and K19-GFP cells. n = 12 (KO), 12 (WT), 9 (K19-GFP); N = 3. Kruskal-Wallis Test, followed by Dunn’s multiple comparison Test; ***, P<0.001. The K19-KO cell sheets show a greater number of fragments compared to WT cell sheets, while K19-GFP cell sheets generate a similar number of fragments as WT. This indicates that elongation of DP strengthens intercellular adhesion in WT and K19-GFP cells.

Techniques: Comparison

Detection of antibodies against ICD proteins in human left ventricular tissue by immunofluorescence analysis. Human left ventricular tissue was incubated with respective AC-IgGs and control IgGs as indicated in the figure. N-CAD was used as a marker for ICDs, and presence of anti-ICD proteins was defined when there was an overlap of IgG staining with N-CAD (white arrows). The yellow arrow in control IgG shows no positive staining of ICDs. Anti-DSG2 antibody was used to show the presence of DSG2 in the cardiac tissue used. AC patients were grouped into DPC (harboring desmoplakin gene mutations) and ARVC (harboring plakophilin2 gene mutations). Images represent immunofluorescence analysis of three repeats. Scale bar: 10 µm

Journal: Cellular and Molecular Life Sciences

Article Title: Catalytic antibodies in arrhythmogenic cardiomyopathy patients cleave desmoglein 2 and N-cadherin and impair cardiomyocyte cohesion

doi: 10.1007/s00018-023-04853-1

Figure Lengend Snippet: Detection of antibodies against ICD proteins in human left ventricular tissue by immunofluorescence analysis. Human left ventricular tissue was incubated with respective AC-IgGs and control IgGs as indicated in the figure. N-CAD was used as a marker for ICDs, and presence of anti-ICD proteins was defined when there was an overlap of IgG staining with N-CAD (white arrows). The yellow arrow in control IgG shows no positive staining of ICDs. Anti-DSG2 antibody was used to show the presence of DSG2 in the cardiac tissue used. AC patients were grouped into DPC (harboring desmoplakin gene mutations) and ARVC (harboring plakophilin2 gene mutations). Images represent immunofluorescence analysis of three repeats. Scale bar: 10 µm

Article Snippet: As a positive control, two different anti-human DSG2 antibodies (DSG2-Origene, #BM5016; DSG2-Abcam, #ab14415) were used at 1:1000 dilutions in blocking buffer.

Techniques: Immunofluorescence, Incubation, Control, Marker, Staining

In vitro DSG2 and N-CAD cleavage assays. A Human DSG2-Fc protein (200 ng/lane) or B N-CAD-Fc protein (200 ng/lane) was incubated with respective IgGs from AC patients (AC1-AC15), water (H 2 O), healthy control IgG (IgG), PV-IgG and AK23 (murine pemphigus monoclonal anti-DSG3 antibody, as a negative control) for 4 h, without (C) and with protease inhibitor (I, cOmplete™), and Western blot analysis was performed. A representative image of three experimental repeats is shown. #Indicates the cleaved fragments. *Indicates mouse IgG heavy chain

Journal: Cellular and Molecular Life Sciences

Article Title: Catalytic antibodies in arrhythmogenic cardiomyopathy patients cleave desmoglein 2 and N-cadherin and impair cardiomyocyte cohesion

doi: 10.1007/s00018-023-04853-1

Figure Lengend Snippet: In vitro DSG2 and N-CAD cleavage assays. A Human DSG2-Fc protein (200 ng/lane) or B N-CAD-Fc protein (200 ng/lane) was incubated with respective IgGs from AC patients (AC1-AC15), water (H 2 O), healthy control IgG (IgG), PV-IgG and AK23 (murine pemphigus monoclonal anti-DSG3 antibody, as a negative control) for 4 h, without (C) and with protease inhibitor (I, cOmplete™), and Western blot analysis was performed. A representative image of three experimental repeats is shown. #Indicates the cleaved fragments. *Indicates mouse IgG heavy chain

Article Snippet: As a positive control, two different anti-human DSG2 antibodies (DSG2-Origene, #BM5016; DSG2-Abcam, #ab14415) were used at 1:1000 dilutions in blocking buffer.

Techniques: In Vitro, Incubation, Control, Negative Control, Protease Inhibitor, Western Blot

Homophilic DSG2 and N-CAD interaction probabilities measured by AFM. A Schematic presentation of an AFM interaction experiment in cell-free conditions. Recombinant DSG2/N-CAD extracellular domain-containing proteins tagged with Fc fragments were covalently linked via a PEG linker to the AFM tip and the mica sheet. A laser is directed to the cantilever tip and reflected onto a photodetector. Each interaction event leads to a deflection of the cantilever, detected by the laser reflected on the photodetector, and a force–distance curve of a specific binding event was produced, together with a topography image and adhesion events. Quantification of DSG2 binding frequency expressed in relative interaction probability. B Desmoplakin-mutated AC patients (DPC), C plakophilin 2-mutated patients (ARVC). D Quantification of N-CAD binding frequency expressed in relative interaction probability in DPC. Each data point represents 1000 curves analyzed across two areas (10 µm × 10 µm). Data are presented as mean ± SEM.* p ≤ 0.05 compared to IgG, and NS is not significant compared to IgG. One-way ANOVA with Holm–Šídák's multiple comparisons test was performed. N = 3–5

Journal: Cellular and Molecular Life Sciences

Article Title: Catalytic antibodies in arrhythmogenic cardiomyopathy patients cleave desmoglein 2 and N-cadherin and impair cardiomyocyte cohesion

doi: 10.1007/s00018-023-04853-1

Figure Lengend Snippet: Homophilic DSG2 and N-CAD interaction probabilities measured by AFM. A Schematic presentation of an AFM interaction experiment in cell-free conditions. Recombinant DSG2/N-CAD extracellular domain-containing proteins tagged with Fc fragments were covalently linked via a PEG linker to the AFM tip and the mica sheet. A laser is directed to the cantilever tip and reflected onto a photodetector. Each interaction event leads to a deflection of the cantilever, detected by the laser reflected on the photodetector, and a force–distance curve of a specific binding event was produced, together with a topography image and adhesion events. Quantification of DSG2 binding frequency expressed in relative interaction probability. B Desmoplakin-mutated AC patients (DPC), C plakophilin 2-mutated patients (ARVC). D Quantification of N-CAD binding frequency expressed in relative interaction probability in DPC. Each data point represents 1000 curves analyzed across two areas (10 µm × 10 µm). Data are presented as mean ± SEM.* p ≤ 0.05 compared to IgG, and NS is not significant compared to IgG. One-way ANOVA with Holm–Šídák's multiple comparisons test was performed. N = 3–5

Article Snippet: As a positive control, two different anti-human DSG2 antibodies (DSG2-Origene, #BM5016; DSG2-Abcam, #ab14415) were used at 1:1000 dilutions in blocking buffer.

Techniques: Recombinant, Binding Assay, Produced

Catalytic antibodies mediated cardiomyocyte cohesion in AC patients. In normal healthy individuals, the cadherin family proteins, desmoglein 2 (DSG2) and N-cadherin (N-CAD) proteins are properly localized to the ICD via the plaque proteins DSP and PKP2 and armadillo proteins PG, α-catenin (α), β-catenin (β) and YAP (Y), respectively. In AC patients, having mutations in either DSP or PKP2 genes (the corresponding proteins are represented with dashed boxes), presence of catalytic antibodies (CA) that can cleave DSG2 or N-CAD could destabilize the cadherin-mediated adhesion (1), which could lead to the activation of p38MAPK (2), which further can lead to desmosome destabilization. Cleavage of cadherin proteins combined with p38MAPK activation could eventually result in reduced cardiomyocyte cohesion (3) and thereby a reduction in the mechanical strengths of the cardiomyocyte. Cartoon created with BioRender.com

Journal: Cellular and Molecular Life Sciences

Article Title: Catalytic antibodies in arrhythmogenic cardiomyopathy patients cleave desmoglein 2 and N-cadherin and impair cardiomyocyte cohesion

doi: 10.1007/s00018-023-04853-1

Figure Lengend Snippet: Catalytic antibodies mediated cardiomyocyte cohesion in AC patients. In normal healthy individuals, the cadherin family proteins, desmoglein 2 (DSG2) and N-cadherin (N-CAD) proteins are properly localized to the ICD via the plaque proteins DSP and PKP2 and armadillo proteins PG, α-catenin (α), β-catenin (β) and YAP (Y), respectively. In AC patients, having mutations in either DSP or PKP2 genes (the corresponding proteins are represented with dashed boxes), presence of catalytic antibodies (CA) that can cleave DSG2 or N-CAD could destabilize the cadherin-mediated adhesion (1), which could lead to the activation of p38MAPK (2), which further can lead to desmosome destabilization. Cleavage of cadherin proteins combined with p38MAPK activation could eventually result in reduced cardiomyocyte cohesion (3) and thereby a reduction in the mechanical strengths of the cardiomyocyte. Cartoon created with BioRender.com

Article Snippet: As a positive control, two different anti-human DSG2 antibodies (DSG2-Origene, #BM5016; DSG2-Abcam, #ab14415) were used at 1:1000 dilutions in blocking buffer.

Techniques: Activation Assay

Journal: bioRxiv

Article Title: Catalytic antibodies in arrhythmogenic cardiomyopathy patients cleave desmoglein 2 and N-cadherin and impair cardiomyocyte cohesion

doi: 10.1101/2023.02.08.527624

Figure Lengend Snippet: Detection of antibodies against ICD proteins in human left ventricular tissue by immunofluorescence analysis. Human left ventricular tissue was incubated with respective AC-IgGs and Control-IgGs as indicated in the figure. N-CAD was used as a marker for ICDs, and presence of anti-ICD proteins was defined when there was an overlap of IgG staining with N-CAD (white arrows). The yellow arrow in control-IgG shows no positive staining of ICDs. Anti-DSG2 antibody was used to show the presence of DSG2 in the cardiac tissue used. AC patients were grouped into DPC (harboring desmoplakin gene mutations) and ARVC (harboring plakophilin2 gene mutations). Images represent immunofluorescence analysis of 3 repeats. Scale bar: 10 µm.

Article Snippet: As a positive control, two different anti-human DSG2 antibodies (DSG2-Origene, #BM5016; DSG2-Abcam, #ab14415) were used at 1:1000 dilutions in blocking buffer.

Techniques: Immunofluorescence, Incubation, Control, Marker, Staining

Journal: bioRxiv

Article Title: Catalytic antibodies in arrhythmogenic cardiomyopathy patients cleave desmoglein 2 and N-cadherin and impair cardiomyocyte cohesion

doi: 10.1101/2023.02.08.527624

Figure Lengend Snippet: In vitro DSG2 and N-CAD Cleavage assays A. Human DSG2-Fc protein (200ng/lane) or B. N-CAD-Fc protein (200 ng/lane) was incubated with respective IgGs from AC patients (AC1-AC15), water (H 2 O), healthy control IgG (IgG), PV-IgG and AK23 (murine pemphigus monoclonal anti-DSG3 antibody, as a negative control) for 4 hours, without (C) and with protease inhibitor (I, cOmplete™) and Western blot analysis was performed. A representative image of 3 experimental repeats is shown. # Indicates the cleaved fragments. * Indicates mouse IgG heavy chain.

Article Snippet: As a positive control, two different anti-human DSG2 antibodies (DSG2-Origene, #BM5016; DSG2-Abcam, #ab14415) were used at 1:1000 dilutions in blocking buffer.

Techniques: In Vitro, Incubation, Control, Negative Control, Protease Inhibitor, Western Blot

Journal: bioRxiv

Article Title: Catalytic antibodies in arrhythmogenic cardiomyopathy patients cleave desmoglein 2 and N-cadherin and impair cardiomyocyte cohesion

doi: 10.1101/2023.02.08.527624

Figure Lengend Snippet: Homophilic DSG2 and N-CAD interaction probabilities measured by AFM A . Schematic presentation of an AFM interaction experiment in cell-free conditions. Recombinant DSG2/N-CAD extracellular domain containing proteins tagged with Fc fragments were covalently linked via a PEG linker to the AFM tip and the mica sheet. A laser is directed to the cantilever tip and reflected onto a photodetector. Each interaction event leads to a deflection of the cantilever, detected by the laser reflected on the photodetector and a force-distance curve of a specific binding event was produced, together with a topography image and adhesion events. Quantification of DSG2 binding frequency expressed in relative interaction probability. B . in desmoplakin mutated AC patients (DPC), C . plakophilin 2 mutated patients (ARVC). D . Quantification of N-CAD binding frequency expressed in relative interaction probability in DPC. Each data point represents 1000 curves analyzed across two areas (10 µm x 10 µm). Data are presented as mean ± SEM.* p ≤ 0.05 compared to IgG, and NS is not significant compared to IgG. One-way ANOVA with Holm-Šídák’s multiple comparisons test was performed. N=3-5.

Article Snippet: As a positive control, two different anti-human DSG2 antibodies (DSG2-Origene, #BM5016; DSG2-Abcam, #ab14415) were used at 1:1000 dilutions in blocking buffer.

Techniques: Recombinant, Binding Assay, Produced

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